Purpose The purpose of this study was to investigate the effects of 3 weeks of fencing specific training on physical fitness in elite fencers. Methods Forty eight elite fencers participated in this program (Male= 24, Female= 24). Training program consists of dynamic stretching, step and agility training, and it was conducted with the general fencing practice during 3 weeks. Body composition and physical fitness (muscle strength/power, agility, anaerobic power and flexibility) were measured before and after training. Data were analyzed using IBM SPSS Statistics ver. 23.0 (IBM Co., Armonk, NY, USA). Paired t-test (pre vs. post) was used for comparison between groups. Results Muscle mass and body fat(%) were significantly changed after training in male group. Agility was significantly improved in change-step jump and reaction time after training both in male and female group. In Anaerobic power, peak power (relative power, absolute power) was significantly increased after training in female group. Flexibility was also significantly improved after training in left ankle ROM of female group. Conclusion Application of fencing specific training program focused on fencing movement seems to be effective on agility in both groups, muscle mass and body fat(%) in male group, and anaerobic power and flexibility in female group.