The purpose of this study was to examine whether the difference between ingestion of nutrition supplements for 8 weeks can regulate physical activities and fatigue recovery. Fifty one middle-aged women participated in this study and were divided into placebo, ingestion of 20g energy supplement and ingestion of 40g energy supplement groups. Energy supplement mainly consists of carbohydrates and proteins. All subjects take in this supplement one time per a day for 8 weeks. Physical activity and fatigue recovery were measured before and after ingestion of energy supplement for 8 weeks by using the Wingate anaerobic and a blood tests. In the Wingate anaerobic test, the peak power(p<.01) and average power(p<.05) were significantly increased in ingestion of 20 and 40g supplement groups compared to the placebo group. Although concentrations of lactate and growth hormone in the blood didn’t show a significant differences among groups, blood concentrations of cortisol and ammonia were further enhanced in ingestion of 20 and 40g supplement groups compared to the placebo group(p<.001). The results of present study provide evidence that energy supplement mixed with carbohydrates and proteins may be effective to increase physical activity as well as to reduce blood concentration of fatigue-related factors after exercise.