Purpose The purpose of this study was to investigate the effect of vibration therapy on the functional, imaging, and mechanical properties in elbow flexor after a single bout eccentric exercise. Methods Twenty-three untrained adults male participated in this study [CON(control)= 11, VT(vibration)= 12]. Volunteers performed 5-set of 6 maximal (90˚/s) eccentric contractions of the non-dominant elbow flexor on a isokinetic dynamometer. Vibration was applied using a side alternating vibratory platform (12 Hz frequency, 4 mm amplitude) with 3-set of 60 sec during 3-day after eccentric exercise. Results The VT group showed a reduction in exercised-induced muscle damage(EIMD) symptoms in the form of attenuating muscle swelling and echo intensity (P<.05) compared with the CON, mechanical properties were improvement (P<.05) compared with the CON. However, muscle soreness, range of motion and maximal voluntary isometric contraction were not significant (P<.05). Conclusion As currently practiced, vibration therapy after eccentric exercise may reduce EIMD by improving quality of the muscle, suggesting that this therapy is an effective strategy for EIMD.