PURPOSE This study examined the biomechanical differences in running shoes with two midsole materials, ethylene-vinyl acetate (EVA) and polyether block amide (PEBA), and carbon fiber plate insertion. METHODS Ten recreational runners participated in the study and performed running trials on a 12m runway at a controlled speed of 3.89 m/s ± 5%. Biomechanical data were obtained for time-continuous variables of the metatarsophalangeal (MTP) joint (angle, moment, and power), as well as for discrete variables (push-off time, peak vertical impact force, peak anterior propulsion force, and timing of joint power transition). Data were analyzed using statistical parametric mapping for continuous data and the Wilcoxon signed-rank test for discrete variables (α = .05). RESULTS Compared with no-plate conditions, the EVA sole with plate significantly reduced push-off time, MTP joint range of motion, positive joint power, and peak anterior propulsion force, with an earlier timing of joint power transition (p < .05). In contrast, the PEBA sole with plate decreased MTP joint range of motion but increased plantar flexion moment, negative joint power, and push-off time (p < .05). Furthermore, under plate-inserted conditions, PEBA significantly increased plantar flexion moment, negative joint power, and push-off time, as well as exhibited a delayed timing of joint power transition compared with EVA (p < .05). CONCLUSIONS The interaction between midsole material and plate insertion causes complex variations in MTP joint energy management. Specifically, EVA shoes with a plate may facilitate rapid roll-off and promote swift turnover, thereby enhancing acceleration. In contrast, PEBA shoes with a plate may promote prolonged energy absorption, which could potentially reduce joint fatigue during long-distance running.