This study was to develop and to apply real-time neurofeedback system for psychological self-regulation of shooters. Neurofeedback system was developed with expert meetings consisting of 8 sport psychology, EEG, and sport engineering experts based on Labview program. Developed neurofeedback system was applied to 4 college shooters for 10 sessions(1 session/week, 30 mins/session). Collected EEG wave data were analyzed by paired sample t-test and independent sample t-test. The results were as follows: Firstly, based on user experience concept neurofeedback system was developed which easily perceived neurofeedback information as traffic lights with minimizing visual search activities. Secondly, after neurofeedback system application right brain activation level of shooters(except one shooter) were increased compared to left brain activation. Based on the results of this study, neurofeedback system can apply various sports and contribute to help athletes’ self-regulation and athletic performance enhancement in sport field.